The inclusion of tress within pastures (i.e., silvopastoral systems) is a nature-positive action to adapt and mitigate climate change, while boosting cattle production and ecosystem services in the Amazon region of Colombia. Deployment of silvopastoral systems often relies on tree species that are commercially available (locally or elsewhere). However, these tree species might not be optimally adapted to local environments. Furthermore, local communities might not be familiar with the use and management of such tree species. These are among factors that threaten the long-term success of silvopastoral systems in the Amazon region. Currently, a project funded by the European Union aims to implement silvopastoral systems in the Amazon region. One component of the project is to deploy silvopastoral systems based on locally collected species. Furthermore, the project aims to co-design silvopastoral systems that are tailored to the farmers’ needs and local context—taking into consideration gender and youth—to ensure large scale deployment and long-term sustainability of such systems. The objective of this presentation is to provide insights of how local knowledge is being used for the deployment of silvopastoral systems in the Amazon region of Colombia.